A mixture of protein is first submitted to an electrophoresis in denaturing condition and. Limits of detection (LODs) in the lower fmol range were reached in the Western blot application using MeCAT and MAXPAR™ as element labeling reagents, whereas even sub-fmol LODs can be achieved in a dot blot experiment for the pure antibodies. Western blot is a procedure to identify and quantify proteins. A Western blot is a polyacrylamide gel electrophoresis methodology that detects bands of proteins specific to HIV antibodies. As a proof-of-principle experiment chemically induced alterations of cytochrome P450 protein expression were investigated and the suitability of the differentially labeled antibodies for Western blot immunoassays of a complex liver microsomal protein fraction was tested. The Western blot protocol provides scientists with a means of comparing relative protein levels in different samples. The purified proteins were eluted by glutathione and subjected to immunoprecipita- tion with anti-HA for HARas followed by Western blot with anti-Raf (left). ELISA also allows quantification of the antigen in question. Western transfer, or Western Blotting, involves protein transfer from polyacrylamide gels to a PVDF or nitrocellulose membrane to allow detection by. Furthermore, the MeCAT labeling strategy was compared with two other commercially available labeling reagents, MAXPAR™ and SCN-DOTA. Like Western blots, ELISA is used to detect peptides in a mixture. Western blotting (WB) is the most widely performed immunoassay and is the best initial validation technique used to identify proteins of interest within a. By using a western blot, researchers are able to identify specific proteins from a complex mixture of proteins extracted from cells. In this paper the lanthanide labeling of antibodies with MeCAT was investigated and the reaction conditions were optimized for application in a Western blot immunoassay analyzed by LA-ICP-MS. Western blotting is an important technique used in cell and molecular biology. We have developed lanthanide labeling strategies for antibodies to adapt conventional biochemical workflows like Western blot immunoassays for detection by laser ablation inductively coupled plasma mass spectrometry ( LA-ICP-MS) analysis with a special interest to apply the multi-element capabilities of ICP-MS for the design of multiplexed immunoassays. One of the most commonly used molecular biology tools, the Western Blot, known also as an immunoblot, allows for investigation of proteins separated by.